FastQCFastQC Report
Thu 2 Jun 2016
DRR024977_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024977_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8462738
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148890.1759359677683511No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118860.14045099824666674No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA87610.10352441491158064No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA150350.045.9049381
GTATCAA255700.041.3519021
TCAACGC338550.030.9651094
ATCAACG339900.030.9404013
TATCAAC350100.030.1202492
CAACGCA385300.027.2311045
AACGCAG396000.026.5635556
ACGCAGA411800.025.0983287
GTACATG391650.024.969051
CGCAGAG425750.024.1209818
TACATGG394950.023.7953472
GCAGAGT444200.022.4313539
ACATGGG412050.022.054293
GTGGTAT79600.019.5502221
AGAGTAC409550.019.52070610-11
CATGGGG302700.018.9624214
CAGAGTA433300.018.68937710-11
GTACTTT214450.018.60632514-15
GAGTACA289350.018.3933031
AGTACTT218150.018.32208812-13
Produced by FastQC (version 0.11.5)