FastQCFastQC Report
Thu 2 Jun 2016
DRR024977_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024977_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8462738
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178050.21039290120998663No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147920.17478976662163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95800.11320213387204No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA157100.046.886551
GTATCAA267900.041.57591
ATCAACG361950.030.4686223
TCAACGC364700.030.2587534
TATCAAC375500.029.5706832
CAACGCA394850.027.9958655
AACGCAG409100.027.0090476
ACGCAGA446100.024.4095297
CGCAGAG461400.023.4981178
GTACATG386300.023.2319871
GCAGAGT480250.022.008369
TACATGG389050.021.9674572
ACATGGG410350.020.3222893
GTGGTAT81050.019.7894651
AGAGTAC443400.019.5553810-11
AGTACTT256000.018.82619912-13
GTACTTT251050.018.6826514-15
GAGTACT255300.018.66611912-13
CAGAGTA469250.018.4530810-11
GAGTACA289900.017.6527921
Produced by FastQC (version 0.11.5)