FastQCFastQC Report
Thu 2 Jun 2016
DRR024976_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024976_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4264315
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87170.2044173565977185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75790.17773077270323603No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44870.10522205793896558No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99400.035.429771
GTATCAA154550.031.883441
ATCAACG176200.027.4035513
TCAACGC177400.027.2169084
TATCAAC182950.026.4965272
CAACGCA184200.026.210625
AACGCAG190550.025.4328636
CGCAGAG205350.023.4150948
ACGCAGA206050.023.4039787
GTACATG95100.022.0509471
GAGTACT132150.021.7456812-13
GCAGAGT222050.021.1881569
AGAGTAC173300.020.77495610-11
TACATGG96250.020.6145762
TCTGTCG10400.020.3373418
CTGTCGC12050.019.5026159
ACATGGG100050.019.2197843
GTACTTT153500.018.61472914-15
AGTACTT146000.018.60452812-13
CAGAGTA220550.017.8266110-11
Produced by FastQC (version 0.11.5)