FastQCFastQC Report
Thu 2 Jun 2016
DRR024976_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024976_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4264315
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95640.22427986675468392No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87150.20437045574728885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54830.12857868145294146No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105650.034.8116721
GTATCAA164300.030.9725781
ATCAACG195150.025.7744183
TCAACGC196700.025.471244
TATCAAC200450.025.1172662
CAACGCA201400.024.876835
AACGCAG207950.024.093266
GTACATG98600.021.9416141
ACGCAGA230800.021.5025397
CGCAGAG230400.021.416988
AGAGTAC192050.021.16670410-11
TACATGG97750.020.5833452
GAGTACT150200.020.4782912-13
AGTACTT162050.019.85081712-13
ACATGGG99200.019.6183433
GCAGAGT245200.019.6067949
TATAACG4001.8189894E-1218.8039442
ACAACGC6300.018.6540493
TACAACG5950.018.1718772
GTACTTT172800.017.63712714-15
Produced by FastQC (version 0.11.5)