FastQCFastQC Report
Thu 2 Jun 2016
DRR024975_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024975_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2808281
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133680.476020740089756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109940.391485040136653No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74550.26546488759493797No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43900.15632338786610028No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36080.1284771716220706No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29320.10440550642902188No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69300.052.1115951
GTATCAA123500.041.7301561
TATAACG3150.038.8115922
TACCTGG29350.034.4452252
TACAACG7000.032.9152152
ATCAACG157450.032.5793233
TCAACGC160100.032.095374
TATCAAC161100.031.9023912
CAACGCA169500.030.5074275
GTGGTAT30750.029.972111
GTACCTG34400.029.7991891
AACGCAG175900.029.6866536
TAACGCA4650.027.2975944
ACAACGC9650.027.2847963
TGGTATC32700.026.8901062
ACCTGGG37000.026.8127083
ACGCAGA197350.026.1961577
ATAACGC4500.026.1208573
CGCAGAG199750.025.6461268
GTACATG133300.025.5043681
Produced by FastQC (version 0.11.5)