FastQCFastQC Report
Thu 2 Jun 2016
DRR024975_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024975_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2808281
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149330.5317487815499945No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127070.45248320947939324No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99050.35270686943365No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42500.15133813176103103No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37220.13253659445048413No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34590.12317143476738973No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74700.051.068251
GTATCAA131700.041.359331
TACCTGG28600.031.5623442
ATCAACG177100.030.4488683
TATCAAC180250.030.0737842
TCAACGC179900.030.0192014
TATAACG2600.028.9321462
CAACGCA188300.028.77995
TACAACG6650.028.2795432
AACGCAG195600.027.8019226
GTACCTG33450.027.4213711
GTGGTAT33350.027.2215081
ACAACGC7900.026.7800713
TGGTATC35050.025.2176072
ACCTGGG35300.025.0385673
ACGCAGA223400.024.1073727
CGCAGAG224750.023.8580198
GTACAAC9600.023.0291181
GTACATG126600.022.924621
ATAACGC3500.022.8353233
Produced by FastQC (version 0.11.5)