FastQCFastQC Report
Thu 2 Jun 2016
DRR024974_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024974_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3550129
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112520.3169462292778657No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95970.27032820497508686No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59180.1666981678693929No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76350.046.682931
GTATCAA135200.038.5355031
ATCAACG164850.031.116513
TCAACGC165600.031.0874024
TATCAAC170500.030.1422312
CAACGCA173250.029.7668865
AACGCAG180150.028.6985916
ACGCAGA200700.025.243477
CGCAGAG203250.024.834278
GTACATG120100.024.2351421
GTGGTAT40400.023.627221
GCAGAGT212750.023.0847079
TACATGG122100.022.6048722
TGGTATC41100.022.19422
TACCTGG24450.021.9232832
ACATGGG126150.021.3561273
AGAGTAC191750.020.51467910-11
GAGTACT139150.020.43253912-13
GTACGCG1200.00505928819.5879425
CAGAGTA211450.019.38132310-11
Produced by FastQC (version 0.11.5)