FastQCFastQC Report
Thu 2 Jun 2016
DRR024974_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024974_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3550129
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128530.3620431820928197No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112760.3176222610502323No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78000.21971032601913904No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85900.044.5745281
GTATCAA146600.036.8994061
ATCAACG184300.028.7728083
TCAACGC187200.028.3457984
TATCAAC189300.028.3116362
CAACGCA194100.027.3623545
AACGCAG200900.026.5520576
GTACATG113200.023.4362561
ACGCAGA228150.023.1322087
CGCAGAG228850.022.8971658
TACCTGG28850.022.6505992
TACATGG115300.022.42562
GCAGAGT241500.021.3280539
GTGGTAT47600.021.0488411
ACATGGG118950.021.0254673
GTACCTG32300.020.8251951
AGAGTAC211350.020.67942410-11
AGTACTT163550.020.34419312-13
GAGTACT160300.019.99441112-13
TGGTATC49050.019.6483252
Produced by FastQC (version 0.11.5)