FastQCFastQC Report
Thu 2 Jun 2016
DRR024973_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024973_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3564931
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108060.30311947131655564No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94550.26522252464353446No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55610.15599179899975624No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71900.052.3811571
GTATCAA131450.040.0616531
ATCAACG159600.032.7272833
TCAACGC160900.032.46154
CAACGCA167600.031.16255
TATCAAC169150.030.9924582
AACGCAG175600.029.711026
TACCTGG29750.026.5506692
ACGCAGA196300.026.337427
CGCAGAG195550.026.2938568
GTGGTAT34650.026.0537011
TGGTATC34050.025.6831912
GTACCTG33650.025.0114821
TATAACG3050.024.6645582
GTACATG106950.024.5315421
ACAACGC6900.024.5291333
GCAGAGT205800.024.3673179
TACATGG105000.023.2397382
ACATGGG108800.021.2168733
GAGTACT145400.021.17024812-13
Produced by FastQC (version 0.11.5)