FastQCFastQC Report
Thu 2 Jun 2016
DRR024973_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024973_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3564931
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118510.3324328016446882No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106870.29978139829354344No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70710.19834886004806265No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73050.053.321651
GTATCAA132150.040.2258071
ATCAACG168650.031.0248533
TCAACGC171350.030.556564
TATAACG3550.030.4600682
CAACGCA177550.029.5160085
TATCAAC178500.029.393912
AACGCAG186700.028.1445856
GTGGTAT32300.026.3614651
GTACATG106500.024.6036221
ACGCAGA211900.024.529297
CGCAGAG211700.024.3970688
ATAACGC4050.024.3761543
TGGTATC33550.024.1027892
TAACGCA4900.023.9798984
TACATGG105550.023.2511082
GCAGAGT223350.022.8088879
TACCTGG28350.022.5536692
ACATGGG109200.021.5252553
GTACCTG31500.021.3559531
Produced by FastQC (version 0.11.5)