FastQCFastQC Report
Thu 2 Jun 2016
DRR024972_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024972_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7734182
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146800.18980675655163015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115820.14975080751914036No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123400.052.4641381
GTATCAA234450.043.3560031
ATCAACG299750.033.579853
TCAACGC303950.033.1298074
TATCAAC318800.031.6336962
CAACGCA340450.029.6316285
AACGCAG353350.028.5858766
ACGCAGA377100.026.4850087
CGCAGAG391600.025.4083188
GTGGTAT52550.025.1406971
GTACATG373700.024.6087471
TACATGG358100.024.2757992
GCAGAGT408850.023.738229
ACATGGG380150.022.4833053
TGGTATC60050.020.7478832
AGAGTAC390900.019.70618410-11
CATGGGG248650.019.284724
GTACTTT208550.019.2562414-15
CAGAGTA407050.019.23607810-11
TAGGTAC17750.019.0624494
Produced by FastQC (version 0.11.5)