FastQCFastQC Report
Thu 2 Jun 2016
DRR024972_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024972_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7734182
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166730.21557548038047206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134130.17342493362581846No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98640.1275377279717493No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126800.053.3841861
GTATCAA242350.043.206911
ATCAACG315700.032.7906233
TCAACGC322600.032.081194
TATCAAC338900.030.658052
CAACGCA349500.029.6254355
AACGCAG363000.028.6006076
GTGGTAT52250.025.7484321
ACGCAGA402050.025.505777
CGCAGAG413400.024.6914988
GTACATG365600.023.6746041
GCAGAGT434600.023.0654589
TACATGG354750.022.8342482
TGGTATC58050.022.0287842
ACATGGG375850.020.9511183
AGAGTAC416400.019.8525410-11
AGTACTT232950.019.2866212-13
CAGAGTA432500.018.96139310-11
GTACTTT231800.018.93626814-15
GAGTACT245350.018.10117112-13
Produced by FastQC (version 0.11.5)