FastQCFastQC Report
Thu 2 Jun 2016
DRR024971_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024971_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4664110
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148650.31871032201213095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118720.25453945125650984No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73680.15797226051701183No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84100.055.6882931
GTATCAA163150.044.52891
ATCAACG209700.034.2545553
TCAACGC211700.033.908374
TATCAAC219450.032.869292
CAACGCA223350.032.1414265
GTGGTAT38300.032.043671
TGGTATC37150.031.5167262
AACGCAG234100.030.8030746
GTACATG203250.027.5075451
ACGCAGA260500.027.2654657
CGCAGAG263150.026.9010038
TACATGG202000.026.5138552
ACATGGG208500.024.9819953
GCAGAGT279650.024.7254779
AGAGTAC263750.021.0294110-11
CATGGGA92800.020.7187754
GAGTACT181000.020.70723512-13
GAGTACA150050.020.682761
CAGAGTA273800.020.54935610-11
Produced by FastQC (version 0.11.5)