FastQCFastQC Report
Thu 2 Jun 2016
DRR024971_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024971_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4664110
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164340.3523501804202731No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138590.297141362446426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91970.1971866015166881No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91600.057.00341
GTATCAA178350.044.4162861
ATCAACG230050.033.9061473
TCAACGC233050.033.4416164
TATCAAC236100.033.256722
CAACGCA242450.032.1256645
AACGCAG253850.030.7755366
GTGGTAT39800.029.3117581
TGGTATC40200.027.3683572
ACGCAGA293350.026.3903647
CGCAGAG294050.026.117498
GTACATG203400.024.9773331
TACATGG202850.024.2214552
GCAGAGT315050.023.9439689
ACATGGG207800.023.0333183
AGAGTAC296200.020.80844310-11
GAGTACT200750.020.56488412-13
AGTACTT208850.020.45358712-13
CAGAGTA307550.020.09410-11
CCGGTCG5450.019.8349889
Produced by FastQC (version 0.11.5)