FastQCFastQC Report
Thu 2 Jun 2016
DRR024970_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024970_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3431347
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82690.24098408001289293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72110.21015070757926846No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41780.12175976373126939No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56450.055.137531
GTATCAA109400.042.1174471
ATCAACG130500.034.7241173
TCAACGC131450.034.435394
CAACGCA139400.032.677195
TATCAAC139850.032.5436442
TGGTATC24250.031.7969462
AACGCAG147500.031.262886
GTGGTAT25550.030.7311531
CGCAGAG160250.028.24718
ACGCAGA162150.028.090467
GCAGAGT173850.025.2532839
TATAACG3250.024.5934162
GTCTCGC10050.024.3271121
TCTGTCG11600.023.5025868
GTACATG96950.022.841591
TACATGG92900.022.5721242
GAGTACT124700.021.18097112-13
GTACCTG26150.021.036211
TACCTGG25050.021.0214942
Produced by FastQC (version 0.11.5)