FastQCFastQC Report
Thu 2 Jun 2016
DRR024970_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024970_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3431347
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88800.2587904983086817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79380.23133772247458506No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50880.14827996119308248No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58700.057.067311
GTATCAA111050.039.570711
TGGTATC23900.034.6203082
GTGGTAT26150.033.8245241
ATCAACG139950.031.0395373
TCAACGC141100.030.7492054
CAACGCA147200.029.634625
TATCAAC147900.029.498232
AACGCAG155000.028.0826786
TATAACG2750.025.6433472
ACGCAGA174750.024.7190747
CGCAGAG173450.024.685398
GTACATG93250.024.2179781
GTACCTG26250.023.8378931
TACATGG90550.023.7789782
ACATGGG92100.022.71523
TACCTGG25650.022.5441532
GCAGAGT186950.022.3245859
TCTGTCG10650.022.0656288
ATAACGC4400.021.3694573
Produced by FastQC (version 0.11.5)