FastQCFastQC Report
Thu 2 Jun 2016
DRR024969_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024969_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3841060
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164860.4292044383581615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133330.34711772271195973No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85290.22204808047778476No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84900.059.0846181
GTATCAA166600.043.739561
ATCAACG213550.033.7643663
TCAACGC216200.033.3931274
TATCAAC221400.032.7222182
CAACGCA228700.031.5897525
GTGGTAT33950.031.1573451
AACGCAG237950.030.4194186
TGGTATC34850.029.8131032
ACGCAGA264850.027.0987537
CGCAGAG268650.026.6629628
GTACATG191300.026.3203811
TACATGG184900.026.1380942
GCAGAGT283800.024.8421469
ACATGGG194100.023.780383
TACCTGG26200.021.3532182
CAGAGTA279100.020.92382810-11
GAGTACT179400.020.8523812-13
CATGGGG90750.020.7691784
AGAGTAC268500.020.62080610-11
Produced by FastQC (version 0.11.5)