FastQCFastQC Report
Thu 2 Jun 2016
DRR024969_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024969_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3841060
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180120.46893305493795984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149310.38872082185646667No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100940.2627920417801336No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40680.10590826490604156No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92800.057.828711
GTATCAA175850.042.1253931
ATCAACG227050.032.381763
TCAACGC229600.031.9983144
TATCAAC235500.031.3799692
CAACGCA238850.030.7787865
AACGCAG249650.029.5037616
GTGGTAT39250.028.759241
TGGTATC40150.027.5152322
ACGCAGA281050.025.8890447
CGCAGAG282100.025.6397238
GTACATG180100.024.6265641
ACAACGC9250.024.3940963
TACATGG175950.024.0727882
GCAGAGT295700.023.9518859
ACATGGG183650.021.7065143
GAGTACT191750.021.06492812-13
AGAGTAC278700.020.84159710-11
AGTACTT192900.020.52518812-13
CAGAGTA292850.020.52460510-11
Produced by FastQC (version 0.11.5)