FastQCFastQC Report
Thu 2 Jun 2016
DRR024968_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024968_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3192316
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101070.31660399534381933No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83250.260782453867349No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49820.15606224446452044No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58250.052.4637341
GTATCAA108950.041.90191
ATCAACG134050.033.559233
TCAACGC135800.033.1964
TATCAAC140200.032.260332
CAACGCA142700.031.623815
GTGGTAT26900.030.7611181
AACGCAG148800.030.5796496
TGGTATC28850.027.2152562
ACGCAGA166100.027.0246357
CGCAGAG168750.026.3491528
TACCTGG25450.026.0479222
GTACATG100450.024.57261
TATAACG2501.6370905E-1124.4481012
GCAGAGT179200.024.2880279
GTACCTG29850.023.7834241
TACATGG100600.023.3208472
ACAACGC8850.022.3086323
ACATGGG105550.021.3771533
AGAGTAC167900.020.56176610-11
Produced by FastQC (version 0.11.5)