FastQCFastQC Report
Thu 2 Jun 2016
DRR024968_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024968_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3192316
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108920.3411942927955754No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93280.29220164921016595No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61980.19415371159997946No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60400.055.6787451
GTATCAA109300.039.8484231
ATCAACG140450.030.9610523
TCAACGC142250.030.4663164
TATCAAC143800.030.0763192
CAACGCA148650.029.2178555
TACCTGG27050.029.0231742
ACAACGC6500.028.9295963
AACGCAG153300.028.4542486
GTGGTAT27400.026.435681
TACAACG8850.025.4972692
TGGTATC27450.025.3463292
ACGCAGA172850.025.0176287
GTACCTG32000.024.6933291
CGCAGAG175200.024.5440738
GTACATG96900.024.318361
TACATGG96000.023.1135432
GCAGAGT185200.022.8635399
ACCTGGG33700.022.0405753
ACATGGG99250.021.0777973
Produced by FastQC (version 0.11.5)