FastQCFastQC Report
Thu 2 Jun 2016
DRR024967_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024967_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3010205
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82720.27479856023094773No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68060.22609755813972804No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42730.1419504651676547No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52150.056.9771041
GTATCAA98200.041.8444251
ATCAACG119600.033.917473
TCAACGC120700.033.6083644
TATCAAC126100.032.39982
CAACGCA126800.031.9550175
AACGCAG132850.030.6402956
ACGCAGA147850.027.4354237
CGCAGAG147050.027.2970098
GTGGTAT21550.026.8346211
TGGTATC21450.026.5213552
GCAGAGT158000.024.7210049
GTACATG82900.023.0822161
TCTGTCG9000.022.4568168
TACATGG82250.022.1214052
CTGTCGC9800.021.5828429
CAGAGTA157400.021.24603710-11
TATAACG3550.021.1899192
TACCTGG21650.020.8473552
ACAACGC5650.020.7986623
Produced by FastQC (version 0.11.5)