FastQCFastQC Report
Thu 2 Jun 2016
DRR024967_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024967_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3010205
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93000.308949058286728No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78620.2611782254032533No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54730.18181485978529702No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51100.057.997571
GTATCAA98450.042.9092371
ATCAACG127100.032.8460583
ACAACGC4600.032.7045363
TCAACGC128750.032.383764
TATCAAC133550.031.435722
CAACGCA134700.031.023095
GTGGTAT20750.029.9258161
AACGCAG140650.029.7775466
TGGTATC20100.029.0029032
ACGCAGA159300.026.172887
CGCAGAG160200.026.0512898
GCAGAGT171500.023.9785379
GTACATG81000.023.8697011
TACATGG80350.022.3508132
AGAGTAC163850.021.39665410-11
ACATGGG81800.020.977583
AGTACTT132050.020.49906212-13
TATAACG4150.020.391082
GAGTACT127600.020.38528812-13
Produced by FastQC (version 0.11.5)