FastQCFastQC Report
Thu 2 Jun 2016
DRR024966_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024966_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5389365
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127910.23733779397016158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104830.19451271160888156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65780.12205519574198444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89750.054.322781
GTATCAA174050.042.9767721
ATCAACG220000.033.396233
TCAACGC224100.032.8259624
TATCAAC231800.031.9248582
CAACGCA234600.031.377685
AACGCAG247000.029.8967676
ACGCAGA272500.026.7708477
CGCAGAG272850.026.615678
GTGGTAT43250.026.0893211
TGGTATC41550.025.9120712
GTACATG208850.025.7981051
TACATGG205100.024.7339482
CGTACCG1151.3768543E-424.5250845
GCAGAGT293500.024.1184679
ACATGGG208000.023.616423
AGAGTAC273700.021.05428510-11
CATGGGG117150.020.1421784
AGTACTT180900.020.05668812-13
GAGTACT176650.019.99382212-13
Produced by FastQC (version 0.11.5)