FastQCFastQC Report
Thu 2 Jun 2016
DRR024966_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024966_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5389365
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144250.26765676475800027No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119470.2216773219108374No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80890.15009189394297845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90700.056.278511
GTATCAA177550.043.1903841
ATCAACG226900.033.4227183
TCAACGC231000.032.8850634
CAACGCA241950.031.4744915
TATCAAC242600.031.4344562
AACGCAG253900.030.0671776
GTGGTAT41900.026.1614631
ACGCAGA289100.026.0471127
CGCAGAG287800.025.9978248
TGGTATC39850.025.3662362
GTACATG203000.024.7047961
GCAGAGT305450.024.049369
TACATGG198500.022.9750842
ACATGGG204800.021.3957653
AGAGTAC292900.020.83421110-11
AGTACTT202750.020.33942812-13
GAGTACT197150.020.2735612-13
CAGAGTA303850.019.71219810-11
TACCTGG40600.019.4549082
Produced by FastQC (version 0.11.5)