FastQCFastQC Report
Thu 2 Jun 2016
DRR024965_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024965_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3337023
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170550.5110842808095719No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136440.40886742464765746No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89340.267723656684416No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37110.11120690507677052No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36570.10958869627209643No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84500.057.1938061
GTATCAA161450.043.037691
TACCTGG25250.036.8651852
ATCAACG204500.033.65183
TCAACGC209050.032.9423454
TATAACG3300.032.766232
TATCAAC213250.032.274882
CAACGCA219800.031.5460075
GTACCGG2550.031.3416141
GTGGTAT33650.030.4567811
AACGCAG231950.030.0534486
TGGTATC34400.028.972772
GTACCTG33200.028.6039431
GTACATG192600.028.2904431
ACCTGGG31500.028.054843
TACATGG187400.027.5451662
CGCAGAG254550.027.2001088
ACGCAGA256050.027.0591227
GCAGAGT265250.025.624419
ACATGGG192700.025.5890583
Produced by FastQC (version 0.11.5)