FastQCFastQC Report
Thu 2 Jun 2016
DRR024965_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024965_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3337023
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187390.561548422051631No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154480.46292758545565915No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107040.3207649452820673No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42020.1259206184674184No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34310.10281619275623811No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33940.10170742005673919No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90050.055.269031
TATAACG3800.043.306792
GTATCAA173700.042.6540871
TACCTGG26100.039.2724042
ATCAACG222900.032.968633
TCAACGC226650.032.3768124
TATCAAC232150.031.757692
GTGGTAT35850.031.7545621
CAACGCA235850.031.1736585
AACGCAG247650.029.7827646
GTACCTG35400.029.6334041
ACCTGGG34050.029.2732373
TGGTATC37800.029.2312622
GTACATG188700.026.9235151
CGCAGAG276700.026.2956318
ACGCAGA279250.026.2438077
TACATGG186750.026.2096772
TAACGCA6950.025.7031124
GTATAAC11000.025.2312771
GTACACG2651.8189894E-1224.8520451
Produced by FastQC (version 0.11.5)