FastQCFastQC Report
Thu 2 Jun 2016
DRR024964_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024964_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2231641
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63610.2850368854130212No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54540.2443941476249988No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33640.15074109142106637No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41000.050.801631
GTATCAA73350.040.318861
TATAACG1353.43789E-1034.8276332
ATCAACG89650.032.403273
TCAACGC90350.032.1529434
CAACGCA93500.031.1206875
TATCAAC94100.031.0783882
AACGCAG96500.030.1518526
TACCTGG18000.026.9043482
TACGCGC700.00883253326.8604096
ACGCAGA107850.026.7607887
CGCAGAG109150.026.3128648
TCTGTCG7750.024.867548
GTACATG55350.024.634181
GTCTCGC6150.024.464291
GCAGAGT117500.024.1229329
GTACCTG21850.023.2396761
TACATGG55950.023.109492
TAACGCA1903.637906E-722.2663634
GTACGGG1303.1530813E-421.7002941
Produced by FastQC (version 0.11.5)