FastQCFastQC Report
Thu 2 Jun 2016
DRR024964_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024964_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2231641
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74120.33213227396341977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63260.28346853279716583No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42670.19120458891013384No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46550.046.4916231
TAACGCA2300.038.8313564
GTATCAA79450.037.069521
TATAACG2350.032.01082
TACCTGG19400.030.7784252
ATCAACG98750.029.4229353
TCAACGC100500.029.0457294
ATAACGC2650.028.38633
CAACGCA102900.028.3682795
TATCAAC102950.028.3145542
GTACCTG21500.028.22851
AACGCAG105800.027.8572756
GTACCGG2203.6379788E-1227.8007951
GTCTCGC6300.026.1374991
TCTGTCG7450.025.2354958
ACAACGC2651.8189894E-1224.838013
ACGCAGA119250.024.6364637
GTGGTAT24150.024.546521
CGCAGAG120700.024.2598938
GTACATG54200.022.6557141
Produced by FastQC (version 0.11.5)