FastQCFastQC Report
Thu 2 Jun 2016
DRR024963_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024963_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3404473
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79290.23289948253371373No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71700.21060528310842824No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39990.1174631139680062No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60700.056.701441
GTATCAA109450.039.994991
ATCAACG134000.032.3816073
TCAACGC135250.032.185664
TATCAAC141300.030.846742
CAACGCA141950.030.7027845
AACGCAG148950.029.2892996
ACGCAGA162700.026.5813037
CGCAGAG162200.026.5473148
GTGGTAT26050.025.4497241
GTACATG95150.024.5594881
GCAGAGT173050.024.4753659
TGGTATC27000.024.3801272
TACATGG91900.023.6884782
ACATGGG95550.021.8450873
GAGTACT122650.021.41993712-13
CAGAGTA174450.021.22049710-11
AGAGTAC161300.021.12903210-11
CTGTCGC7850.020.9591859
TCTGTCG7650.020.8926458
Produced by FastQC (version 0.11.5)