FastQCFastQC Report
Thu 2 Jun 2016
DRR024963_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024963_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3404473
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86620.2544299807929157No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79120.23240013946358218No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46570.13679062809427478No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62750.055.1738971
GTATCAA112150.039.8048971
ATCAACG138750.031.7817443
TCAACGC139750.031.5492274
CAACGCA145300.030.4735455
TATCAAC145400.030.4584032
TATAACG3400.030.4203622
AACGCAG152900.029.020326
GTGGTAT26900.028.329071
TGGTATC27200.027.1363472
CGCAGAG171850.025.5980328
ACGCAGA173650.025.4969547
GCAGAGT184400.023.3461279
GTACATG93100.022.9895941
TACATGG90600.022.6244962
ACATGGG91750.021.3154833
ATAACGC4450.021.1289483
AGAGTAC172500.020.7590710-11
GAGTACT133450.020.60027112-13
CAGAGTA184150.020.40275210-11
Produced by FastQC (version 0.11.5)