FastQCFastQC Report
Thu 2 Jun 2016
DRR024962_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024962_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3336428
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184100.5517877202804916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142690.4276729484346733No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98630.2956155505228945No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43460.13025906748174992No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37070.11110684840194363No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35820.10736032667271705No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90100.057.604551
GTATCAA174950.042.511361
TACCTGG30450.041.0683062
TATAACG3550.035.7559132
ACCTGGG34900.034.345923
GTACCTG37400.033.436631
ATCAACG227700.032.4526333
TCAACGC231550.031.9338174
TATCAAC240900.030.8342062
GTGGTAT36050.030.3852711
CAACGCA244150.030.364175
TGGTATC35700.029.8930442
AACGCAG255850.029.1584846
GTACATG202050.027.6885741
TACATGG196700.027.3422432
ACGCAGA281250.026.256157
CGCAGAG280950.026.1164868
TACAACG8400.025.1852172
ACATGGG207100.025.0127773
GCAGAGT294400.024.5082029
Produced by FastQC (version 0.11.5)