FastQCFastQC Report
Thu 2 Jun 2016
DRR024962_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024962_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3336428
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206610.6192550835804039No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168180.5040720195370617No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120200.3602655294824285No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42670.12789126574887874No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39180.1174309770808781No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37820.11335476143947959No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95600.055.8601761
GTATCAA183550.041.4751971
TACCTGG26750.038.493292
TATAACG4500.037.614452
GTACCGG2850.036.319691
ACCTGGG29700.034.0366443
ATCAACG239350.031.6072943
GTGGTAT37950.030.9950681
TCAACGC243800.030.9853024
GTACCTG33700.030.8550831
TATCAAC250050.030.3111822
TACAACG7650.030.1161442
CAACGCA253450.029.8982875
TGGTATC38850.029.651032
AACGCAG265850.028.6275226
ACAACGC9850.025.7763983
GTACATG200500.025.6255151
GTACACG2950.025.5189191
ACGCAGA295800.025.4417617
CGCAGAG295100.025.3067938
Produced by FastQC (version 0.11.5)