FastQCFastQC Report
Thu 2 Jun 2016
DRR024961_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024961_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4506398
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100560.22314939781173349No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86100.19106168607388874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50460.11197413100218845No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71200.051.972661
GTATCAA131500.041.1556781
ATCAACG158000.033.650333
TCAACGC160450.033.0757034
TATCAAC167700.031.823142
CAACGCA167500.031.7972265
AACGCAG174250.030.6177396
ACGCAGA194750.026.887367
CGCAGAG195200.026.5842768
GTGGTAT34350.025.8711221
GCAGAGT213000.023.7668539
GTACATG123300.023.2619951
TGGTATC36250.022.1803462
TACATGG123350.021.651572
GAGTACT141000.020.69915612-13
AGAGTAC195700.020.39142810-11
TACCTGG35450.020.2934272
ACATGGG126150.020.1974163
CAGAGTA209150.020.17006910-11
AGTACTT147650.019.3849212-13
Produced by FastQC (version 0.11.5)