FastQCFastQC Report
Thu 2 Jun 2016
DRR024961_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024961_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4506398
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112180.24893495869650217No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98730.21908850483246264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62650.13902456019197595No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70000.054.9012071
GTATCAA130650.041.152351
ATCAACG164600.032.04683
TCAACGC166050.031.6498374
TATCAAC171250.030.9128512
CAACGCA171800.030.6452675
AACGCAG178800.029.4192186
GTGGTAT31450.027.3708521
ACGCAGA203050.025.7190867
CGCAGAG204550.025.3897888
GTACATG129200.024.3204611
TGGTATC33100.023.0100882
GCAGAGT222650.022.8191389
TACATGG129050.022.7694872
ACATGGG129850.021.6511423
AGAGTAC208650.020.29428710-11
GAGTACT153700.019.7059412-13
CAGAGTA221450.019.3228710-11
TACCTGG33050.018.6350732
AGTACTT162300.018.61832212-13
Produced by FastQC (version 0.11.5)