FastQCFastQC Report
Thu 2 Jun 2016
DRR024960_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024960_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3488141
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90440.2592785096703373No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78660.22550693908302447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44960.12889387212271522No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54150.056.95291
GTATCAA101650.042.364161
ATCAACG123500.034.3696563
TCAACGC124600.034.0275344
TATCAAC129700.032.8759082
CAACGCA131200.032.3883675
AACGCAG136850.031.153336
GTGGTAT24450.027.4958821
ACGCAGA156150.027.122197
CGCAGAG156050.027.0190878
TGGTATC24500.026.4803332
GTACATG84550.025.4660951
TACATGG83950.024.75212
GCAGAGT168600.024.2551339
ACATGGG85150.023.5720653
ATAACGC2602.7284841E-1123.5030613
GTCTCGC9750.022.6622851
TACCTGG27050.021.724672
TCTGTCG9950.021.258448
CAGAGTA167050.021.01859510-11
Produced by FastQC (version 0.11.5)