FastQCFastQC Report
Thu 2 Jun 2016
DRR024960_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024960_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3488141
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100310.2875743841777038No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89910.25775907567956685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55800.1599705975188503No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58950.054.8156281
GTATCAA107950.040.0863651
ATCAACG135100.031.5625173
TCAACGC136200.031.3040164
TATCAAC140250.030.60512
CAACGCA141800.030.0677495
AACGCAG148400.028.762186
GTGGTAT27550.027.8289951
TGGTATC28000.026.3613432
ACGCAGA168750.025.0418877
CGCAGAG169250.024.9928178
GTACATG86450.024.211771
TATAACG3200.023.506932
GCAGAGT182600.022.7022659
TACATGG88350.022.4027582
ACATGGG88850.021.5355853
TCTGTCG9050.020.773658
AGAGTAC170650.020.73762110-11
GTACACG3202.0008883E-1120.5783021
TACCTGG26800.020.5247082
Produced by FastQC (version 0.11.5)