FastQCFastQC Report
Thu 2 Jun 2016
DRR024959_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024959_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3181309
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86070.27054900985726316No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71750.22553609221864332No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43160.1356674249499184No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52000.052.3433951
GTATCAA96750.041.8835031
ATCAACG119900.032.8903123
TCAACGC121050.032.5773354
TATCAAC126250.031.6867732
CAACGCA126750.031.224555
AACGCAG133200.029.8875836
TACCTGG26850.026.962262
ACGCAGA148350.026.39187
ACAACGC6450.026.2342133
CGCAGAG148400.026.1612058
GTGGTAT25250.025.320031
GTACCTG30050.025.1865141
GCAGAGT159000.023.7076689
GTACATG75650.023.6135541
TGGTATC25650.023.092082
GTCTCGC9800.023.0251161
TACAACG7550.022.4148272
CTGTCGC10800.021.7599479
TACATGG77800.021.6313342
Produced by FastQC (version 0.11.5)