FastQCFastQC Report
Thu 2 Jun 2016
DRR024959_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024959_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3181309
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96180.3023283811789424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83530.26256487502471465No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54470.17121882847595124No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56150.054.9586681
GTATCAA103150.040.0412331
ATCAACG128650.031.4626623
TCAACGC129600.031.300154
GTGGTAT25300.030.8652441
TATCAAC133150.030.6111762
CAACGCA135200.030.073235
TACCTGG26900.029.010682
ACAACGC5700.028.8665753
AACGCAG142100.028.645586
GTACCTG28950.027.2987561
TGGTATC26350.027.2968732
TCTGTCG9550.026.081838
TATAACG2900.025.937262
ACGCAGA160800.025.196577
CGCAGAG160400.025.1683278
TACAACG6750.025.0726832
ATAACGC3100.024.2638873
GTCTCGC8150.023.0879041
GCAGAGT171050.023.0792549
Produced by FastQC (version 0.11.5)