FastQCFastQC Report
Thu 2 Jun 2016
DRR024958_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024958_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4184468
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97880.23391265030584535No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82510.19718157720407947No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51870.12395841000576419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85800.045.8092541
GTATCAA146550.036.7648661
ATCAACG176700.029.820153
TCAACGC178550.029.5111774
TATCAAC186750.028.447682
CAACGCA186800.028.4111615
AACGCAG194350.027.35426
ACGCAGA215000.024.486147
CGCAGAG214450.024.2859138
GTACATG131700.024.2748931
TACATGG128600.023.4705412
GCAGAGT230850.022.0922859
ACATGGG131250.022.0250323
AGAGTAC202050.020.61178610-11
GAGTACT143850.020.53401612-13
GTACCTG34250.020.3158651
TACCTGG32450.019.4141852
AGTACTT151250.019.26525712-13
ATAACGC5150.019.166943
TAACGCA5000.018.8018594
Produced by FastQC (version 0.11.5)