FastQCFastQC Report
Thu 2 Jun 2016
DRR024958_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024958_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4184468
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111530.26653328451788855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93560.22358875728049538No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66790.15961407758405607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92050.045.2695921
GTATCAA159850.037.8377231
ATCAACG200350.030.1037863
TCAACGC203100.029.646344
TATCAAC207750.029.054472
CAACGCA210350.028.6468855
AACGCAG218200.027.5731966
CGCAGAG241400.024.4535948
ACGCAGA243050.024.4437477
GCAGAGT256400.022.5830739
ACAACGC7300.021.8947433
GTACATG128250.021.7467331
AGAGTAC227000.021.66582110-11
TACATGG123100.021.5764182
AGTACTT173250.020.69690112-13
GAGTACT164200.020.39227712-13
ACATGGG128500.019.8280473
TGGTATC50350.019.1400642
TACCTGG31400.018.7141152
CAGAGTA257300.018.69432810-11
Produced by FastQC (version 0.11.5)