FastQCFastQC Report
Thu 2 Jun 2016
DRR024957_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024957_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4295651
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108420.2523948058163943No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91920.21398386414538798No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53560.12468424459994538No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71950.054.5647161
GTATCAA132150.041.5914041
ATCAACG160650.033.8269273
TCAACGC161800.033.5845454
CAACGCA169450.032.0694545
TATCAAC170250.031.9523262
AACGCAG179600.030.4915666
GTACATG142200.027.4431971
ACGCAGA199600.027.1295137
CGCAGAG201350.026.6135758
TACATGG139550.026.2797362
ACATGGG140900.024.8226053
GCAGAGT213300.024.681829
GTGGTAT32250.024.6384411
TGGTATC32150.023.6913742
GAGTACT145700.021.3217912-13
CAGAGTA213950.020.76177610-11
AGAGTAC202650.020.5393610-11
CATGGGG81600.020.219814
GAGTACA107500.019.8128051
Produced by FastQC (version 0.11.5)