FastQCFastQC Report
Thu 2 Jun 2016
DRR024957_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024957_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4295651
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119720.2787004810213865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105320.2451782046539628No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66720.1553198805023965No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73050.055.704451
GTATCAA134850.041.6182171
ATCAACG168450.032.9315073
TCAACGC170100.032.582914
CAACGCA176450.031.3570465
TATCAAC178250.031.2268262
AACGCAG185150.030.0098636
GTGGTAT32150.028.2403661
TGGTATC31950.027.809722
ACGCAGA213200.025.7954837
GTACATG138600.025.7615831
CGCAGAG213950.025.5052478
TACATGG135350.024.7649212
GCAGAGT228200.023.4594429
ACATGGG138950.023.2771853
GAGTACT157350.021.11575712-13
AGAGTAC216550.020.10671810-11
CAGAGTA225350.020.04101810-11
AGTACTT164650.019.55162412-13
CATGGGG80500.019.5043284
Produced by FastQC (version 0.11.5)