FastQCFastQC Report
Thu 2 Jun 2016
DRR024956_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024956_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3105848
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75570.24331519121347858No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64290.20699660768975173No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38420.12370212579623986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52400.050.6081771
GTATCAA88900.039.3499371
TCAACGC108150.031.9898534
ATCAACG109250.031.6682663
TATCAAC112250.030.8293462
CAACGCA113200.030.6052555
TACCTGG28500.030.3560892
AACGCAG118050.029.624226
GTACATG103650.026.9457341
GTACCTG34800.025.6712631
ACGCAGA134950.025.6701017
GTGGTAT22600.025.5899521
TACATGG104250.025.0316682
ACCTGGG33200.024.7780953
CGCAGAG139800.024.5105658
ACATGGG104850.023.6719173
TAACGCA3350.022.4510214
GCAGAGT150350.022.4155149
TGGTATC25350.020.9591332
CAGAGTA144700.019.99366810-11
Produced by FastQC (version 0.11.5)