FastQCFastQC Report
Thu 2 Jun 2016
DRR024956_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024956_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3105848
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88280.2842379923293091No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75040.2416087329450765No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49050.15792788314173778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55150.050.4968261
GTATCAA94200.038.802351
TACCTGG28000.033.5848162
ATCAACG116000.030.9670183
TCAACGC116650.030.9084134
CAACGCA121300.029.8398085
TATCAAC121600.029.7347152
AACGCAG125550.028.867146
ACAACGC5250.027.7630023
GTGGTAT22450.027.6595731
GTACCTG36650.026.5695551
GTACATG99550.025.7066541
ACGCAGA147000.024.4630247
TACATGG99550.024.1824152
ACCTGGG37850.023.8506013
TACAACG6550.023.6888312
CGCAGAG151900.023.6085828
ACATGGG101250.022.7543833
TGGTATC26100.022.3384062
GCAGAGT162100.021.5141439
Produced by FastQC (version 0.11.5)