FastQCFastQC Report
Thu 2 Jun 2016
DRR024955_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024955_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5004490
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103040.20589510619463725No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85360.17056683098577477No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50920.10174862973050201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74550.050.3271751
GTATCAA139150.041.3904571
ATCAACG167700.033.6923983
TCAACGC170400.033.2127154
CAACGCA178700.031.6713665
TATCAAC180450.031.5265052
AACGCAG186500.030.4208686
ACGCAGA209100.026.9744767
CGCAGAG209600.026.7755788
GTACATG136100.025.321721
GCAGAGT230000.023.8080529
TACATGG137450.023.6705672
TGGTATC38650.022.7477992
GTGGTAT40400.022.5770761
ACATGGG138450.022.2386723
GAGTACT149500.021.17229712-13
AGAGTAC208300.021.00786210-11
CAGAGTA226650.019.7736310-11
AGTACTT160000.019.68005812-13
GTACCTG39900.019.0892751
Produced by FastQC (version 0.11.5)