FastQCFastQC Report
Thu 2 Jun 2016
DRR024955_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024955_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5004490
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111860.2235192796868412No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97180.19418562131206177No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61760.12340917855765522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80400.051.260691
GTATCAA143550.039.820751
ATCAACG179550.031.5032983
TCAACGC182200.030.9621094
TATCAAC190050.029.8620452
CAACGCA190000.029.7157765
AACGCAG197450.028.6421896
TATAACG4800.025.4690252
ACGCAGA225100.024.8713447
CGCAGAG227600.024.3677988
GTACATG133450.023.3033981
GTGGTAT42050.023.0482311
TAACGCA5750.022.8922184
GCAGAGT246050.022.3113579
TACATGG135200.021.6318592
TGGTATC40600.021.5410612
ATAACGC5750.021.2608853
ACATGGG137400.020.4982093
GAGTACT163400.020.08898512-13
AGAGTAC224500.020.0850210-11
Produced by FastQC (version 0.11.5)