FastQCFastQC Report
Thu 2 Jun 2016
DRR024954_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024954_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3641065
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88050.24182485069615622No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76500.21010336261505905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43430.11927828808329431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61450.056.6972351
GTATCAA114650.042.281481
ATCAACG139350.034.3085173
TCAACGC140550.034.0815544
TATCAAC145800.033.183592
CAACGCA147250.032.5964555
AACGCAG154200.031.3685996
TGGTATC27300.030.656522
GTGGTAT29250.030.2202111
ACGCAGA169700.028.0044777
CGCAGAG171800.027.3334548
GCAGAGT183550.024.969079
ATAACGC4000.023.5048773
GTACATG96400.022.9237881
TAACGCA4500.021.9372834
TACATGG97100.021.5963922
CCGGTCG3051.0913936E-1121.576479
GAGTACT128650.021.48142212-13
CAGAGTA181750.021.4274410-11
AGAGTAC173300.020.64135710-11
Produced by FastQC (version 0.11.5)