FastQCFastQC Report
Thu 2 Jun 2016
DRR024954_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024954_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3641065
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100140.27502942133688907No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88970.24435158394590595No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52700.14473787202370736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63150.056.3951
GTATCAA117700.041.44961
ATCAACG148500.032.3556443
TCAACGC150550.031.9427834
TATCAAC156050.030.9417022
CAACGCA156650.030.6389055
GTGGTAT29250.030.3986281
AACGCAG163050.029.5515986
TGGTATC28600.028.932382
ACGCAGA185950.025.7089757
CGCAGAG185350.025.6379178
GCAGAGT202200.023.1065659
GTACATG99600.020.6886841
AGAGTAC190500.020.35249110-11
GAGTACT142650.020.34313212-13
ACAACGC7000.020.1487853
CAGAGTA200550.019.87155210-11
TACAACG6950.019.6178192
TACCTGG28800.019.5896342
GTACCTG30400.019.3443661
Produced by FastQC (version 0.11.5)