FastQCFastQC Report
Thu 2 Jun 2016
DRR024953_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024953_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3258263
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88070.27029739465475927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75440.2315344095918592No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45310.13906182527315933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72750.040.4548951
ACAACGC6950.035.1707533
GTATCAA117350.035.0954481
TACCTGG31150.033.808022
GTACCTG33850.032.361381
TAGACCG1201.651697E-731.3380415
ATCAACG140950.028.5477433
TCAACGC141850.028.4987324
TACAACG8400.027.9846882
TATCAAC147000.027.6328812
CAACGCA148050.027.4009425
AACGCAG153700.026.6069536
TATAACG3950.026.1851672
ATAACGC3950.026.1811473
GTCTCGC10700.025.923761
TCTGTCG10750.024.9235298
GTACATG83450.024.4508041
GTACAAC11250.024.238471
ACGCAGA171750.023.5096517
CGCAGAG170850.023.468068
Produced by FastQC (version 0.11.5)