FastQCFastQC Report
Thu 2 Jun 2016
DRR024953_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024953_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3258263
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97830.3002520054397082No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84120.2581743708227359No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56830.174418087183263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGC5550.041.5054323
GGTATCA80050.036.8994751
TACCTGG31900.035.0753022
TACAACG6600.033.4787372
GTACCTG34750.032.620011
GTATCAA125400.032.481961
TATAACG3850.029.3066142
ATCAACG152950.026.556213
TCAACGC154100.026.2949964
TATCAAC158650.025.6621462
CAACGCA159000.025.484655
GTACATG81850.024.6524731
AACGCAG165750.024.5886156
ATAACGC4400.024.5740623
GTACCGG2352.0008883E-1024.0179141
TACATGG81500.023.5928752
GTCTCGC10850.023.4091641
ACCTGGG45300.023.1424063
TAACGCA5350.021.9661884
CGCTCTG11600.021.8828685
Produced by FastQC (version 0.11.5)